2/27/2024 0 Comments Mouse mesenchymal stem cells![]() Here, we studied the effects of oxygen concentrations and initial medium replacement intervals, along with cell passages, on mouse BM-MSCs isolated with differential adhesion method. However, systematic report on comparison of characteristics in primary BM-MSCs between different culture conditions is rare. Several techniques have been reported to improve the purity and in vitro growth of mouse BM-MSCs. The lack of specific mouse BM-MSC markers increases the difficulty. 2012 2012:948098.In vitro culture of mesenchymal stem cells (MSCs) from mouse bone marrow (BM) has been hampered because of the low yield of MSCs during isolation and the contamination of hematopoietic cells during expansion. Macrophages in tumor microenvironments and the progression of tumors. Hao NB, Lu MH, Fan YH, Cao YL, Zhang ZR, Yang SM. Mouse bone marrow-derived mesenchymal stromal cells turn activated macrophages into a regulatory-like profile. Maggini J, Mirkin G, Bognanni I, Holmberg J, Piazzon IM, Nepomnaschy I, et al. Human MSC suppression correlates with cytokine induction of indoleamine 2,3-dioxygenase and bystander M2 macrophage differentiation. Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury. Nakajima H, Uchida K, Guerrero AR, Watanabe S, Sugita D, Takeura N, et al. Macrophages modulate the viability and growth of human mesenchymal stem cells. LVEF, left ventricular ejection fraction.įreytes DO, Kang JW, Marcos-Campos I, Vunjak-Novakovic G. Pink arrowheads indicate injected MSCs white arrowheads indicate macrophages with Arg1 expression. In the high-magnification view of the rectangle, Arg1-expressing CD68 (+) macrophages (yellow) near DAPI-labeled MSCs (blue) were observed in the infarct zone. Representative images showed immunofluorescent staining for the macrophage marker CD68 (green) and Arg1 (red) in the infarct myocardium. ( b) Immunocytochemistry images of 4′,6-diamidino-2-phenylindole (DAPI)-labeled MSCs or PBS injected in the hearts of infarct rats at 7 days post-cell injection. The left ventricular ejection fraction was better in the MSC group compared with the PBS group. ( a) Representative images showed that the fibrotic area was smaller in the mesenchymal stem cell (MSC) group compared with the phosphate-buffered saline (PBS) group. The identification of arginase-1 (Arg1)-expressing macrophages in infarct myocardium. These results suggest that the preferential shift of the macrophage phenotype from M1 to M2 may be related to the immune-modulating characteristics of MSCs that contribute to cardiac repair. Specifically, the ratio of iNOS to Arg1 in BMDMs was notably downregulated by co-culturing with MSCs. In contrast, the M2 markers such as IL-10, IL-4, CD206 and Arg1 were markedly increased by co-culturing with MSCs. In BMDMs co-cultured with MSCs, the M1 markers such as interleukin-6 (IL-6), IL-1β, monocyte chemoattractant protein-1 and inducible nitric oxide synthase (iNOS) were significantly reduced. In the MSC-injected myocardium, the macrophages adjacent to the MSCs showed strong expression of arginase-1 (Arg1), an M2 marker. MSCs were injected within the infarct myocardium, and we analyzed the phenotype of the infiltrated macrophages by immunostaining. For animal studies, MI was induced by the ligation of the rat coronary artery. To determine the macrophage phenotype, classical M1 markers and alternative M2 markers were analyzed with or without co-culturing with MSCs in a transwell system. MSCs isolated from bone marrow and bone marrow-derived macrophages (BMDMs) underwent differentiation induced by macrophage colony-stimulating factor. This study was designed to assess the functional relationship between the macrophage phenotype and MSCs. We hypothesized that MSCs may modulate the immunologic environment to accelerate regeneration. During myocardial infarction (MI), infiltrated macrophages have pivotal roles in inflammation, angiogenesis and cardiac remodeling. Mesenchymal stem cells (MSCs) have been widely studied for their applications in stem cell-based regeneration.
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